HPV testing for cervical cancer screening

PCR

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Human Papillomaviruses typically infect the skin and mucosal surfaces. There are more than 200 different types: approximately 100 of them are fully sequenced and 40 types are known to infect the anogenital mucosa of humans. The highest rates of newly acquired HPV infections are observed in young women, peaking at the age soon after the onset of sexual activity. HPV types detected in the anogenital tract are subdivided into lowrisk and high-risk types, based on their relative risk to cause the rare complication such as neoplasia. Low-risk HPV types are typically found in low-grade squamous intraepithelial lesions as well as anogenital warts. The high-risk HPVs are found in low- and high-grade lesions, such as cancers of cervix and other types of cancer in anogenital sites (vulva, vagina, anus).

HPV types frequently detected in the anogenital tract

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HPV testing relies on molecular methods

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  • HPV testing is incorporated into cervical cancer screening in various combinations with Papanicolaou (Pap) test.
  • Polymerase chain reaction an assay can be used for primary screening and genotyping of high-risk HPV-types.
  • HPV DNA detection is a negative predictor. Current research is investigating the degree of impact on unknown-risk HPV types on clinical important outcomes.



The RealBest assay kits for diagnostics of HPV

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Features of RealBest kits

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  • Easy-to-use: just add a sample of eluted DNA into 0.2 ml white tubes with Lyophilized Ready Master Mix;
  • Reliability of analysis: kit contains Noncompetitive Internal Control sample;
  • High sensitivity: 100 copies (genome-equivalents) per sample;
  • Universal and rapid amplification protocol for all infections;
  • Convenience of storage and transportation: no freezing required, transportation up to 26 °C for no more than 10 days is acceptable