Single nucleotide polymorphisms and mutations in human genes

PCR

RealBest-Genetics kits are designed for various diagnostic areas

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PCR analysis using RealBest-Genetics kits

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Sample types

  • Buccal swabs (in Transport solution, for example No8894)
  • Whole blood

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DNA extraction kits

Manual extraction:
8845 RealBest-Genetics DNA-express

Automated DNA extraction (KingFisher Flex, Auto-Pure 96, RbMag):
8846 RealBest GenMag

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RealBest-Genetics Kits for mutations detection

Methods: asymmetric PCR with following melting curve analysis of PCR products or real-time PCR for allele detection

Multiplex PCR: detection up to three targets in one test tube – each target is detected by specific fluorescent channel; different alleles result in different melting temperatures

Easy-to-use: Lyophilized Ready Master Mix in 48/96-tube plates – just add a sample of extracted DNA into tube

PCR cyclers: kits are compatible with plate-type real-time PCR cyclers CFX96 (Bio- Rad, USA), Gentier 96E/R (Xi’an TianLong, S&T Co., Ltd., China) and others with similar characteristics after validation

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Analysis of results

GP.RealBest Diagnostics software for convenient workflow and interpretation of results